Medium for preparing feeder cells for embryonic stem cells...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/0735 (2010.01) C12N 5/071 (2010.01) A61K 35/36 (2006.01) A61K 35/48 (2006.01)

Patent

CA 2555021

It is intended to efficiently establish a feeder cell usable in culturing embryonic stem cells including human embryonic stem cells from a restricted donor-origin material to thereby provide a medium for preparing feeder cells for embryonic stem cells enabling culture with little risk of infection. It is also intended to provide a method of producing feeder cells which are relatively safe in the case of culturing together with embryonic stem cells including human embryonic stem cells, and feeder cells thus obtained. By using a medium for preparing feeder cells for embryonic stem cells obtained by adding at least serum albumin and insulin to a basal medium, it is possible to culture, in a stable state, a mass of cells including at least one type of cells available as feeder cells for embryonic stem cells which are selected from among fetal epidermal fibroblasts, fetal muscular fibroblasts, fetal pulmonary fibroblasts, fetal epithelial cells, fetal endothelial cells, adult epidermal fibroblasts, adult pulmonary fibroblasts, adult epithelial fibroblasts and adult epidermal fibroblasts.

: L'intention est d'établir efficacement une cellule nourricière utilisable pour la culture des cellules souches embryonnaires y compris des cellules souches embryonnaires humaines à partir d'un matériau d'origine donneur restreinte pour fournir ainsi un moyen de préparer des cellules nourricières pour des cellules nourricières embryonnaires permettant la culture avec un léger risque d'infection. Il est également prévu de fournir une méthode de production des cellules nourricières qui sont relativement sûres dans le cas de culture avec des cellules souches embryonnaires comprenant des cellules souches embryonnaires humaines, et des cellules nourricières ainsi obtenues. En utilisant un moyen de préparer des cellules nourricières pour des cellules souches embryonnaires obtenues en ajoutant au moins un sérum-albumine et de l'insuline à un milieu de base, il est possible de cultiver, dans un état stable, une masse de cellules comprenant au moins un type cellules disponibles comme cellules nourricières pour des cellules souches embryonnaires qui sont sélectionnées parmi des fibrocytes épidermiques foetaux, des fibrocytes musculaires foetaux, des fibrocytes pulmonaires foetaux, des cellules épithéliales foetales, des cellules endothéliales foetales, des fibrocytes épidermiques adultes, des fibrocytes pulmonaires adultes, des fibrocytes épithéliales adultes et des fibrocytes épidermiques adultes.

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