Medium for the separation of human blood leucocytes

Details Medium for the separation of human blood leucocytes Medium for the separation of human blood leucocytes

A - Human Necessities

61

K

167/114

A61K 35/14 (2006.01) B01D 43/00 (2006.01)

Patent

CA 1127537

ABSTRACT OF THE DISCLOSURE A rapid one-step density separation method for sep- arating and purifying whole blood into mononuclear and polymorphonuclear leucocyte and erythrocyte fractions wherein use is made of a separating medium consisting of a single aqueous solution of a high molecular weight sucrose or glucose polymer, and a water soluble metrizoate or diatrizoate compound, the density of the solution being greater than 1.095 gm/ml at room temperature and the concentration of the sucrose or glucose polymer lying in the range of 5 to 10% (based on weight/total volume of solution).

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