Meganuclease variants cleaving a dna target sequence from a...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/22 (2006.01) C12N 15/55 (2006.01) A61K 48/00 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2639149

An I-CreI variant which has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG core domain situated from positions 26 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from a xeroderma pigmentosum gene. Use of said variant and derived products for the prevention and the treatment of Xeroderma pigmentosum.

L'invention concerne un variant I-CreI possédant au moins deux substitutions, une dans chacun des deux sous-domaines fonctionnels du domaine central LAGLIDADG allant des positions 26 à 40 et 44 à 77 de I-CreI, ledit variant étant capable de couper une séquence d'ADN cible d'un gène de Xeroderma pigmentosum, ainsi que l'utilisation dudit variant et de produits dérivés pour la prévention et le traitement de Xeroderma pigmentosum.

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