Membrane assay method

Details Membrane assay method Membrane assay method

G - Physics

01

N

G01N 33/50 (2006.01)

Patent

CA 2639414

The present invention provides an assay method for a cell-containing body sample, said method comprising treating said sample under conditions whereby to cause cell lysis, preferably by means of a detergent; and subjecting the thus-generated lysed sample to conditions causing the cleavage of nucleic acid molecules. The invention additionally provides the use of nucleic acid cleavage conditions in enhancing a membrane assay, a device for carrying out such an assay, and a kit for use in the assay.

La présente invention concerne un procédé de dosage pour un échantillon corporel contenant des cellules, ledit procédé comprenant les étapes consistant à traiter ledit échantillon dans des conditions par lesquelles la lyse des cellules est provoquée, de préférence au moyen d'un détergent; et à soumettre l'échantillon lysé ainsi généré à des conditions provoquant le clivage des molécules d'acides nucléiques. L'invention concerne de plus l'utilisation de conditions de clivage des acides nucléiques pour améliorer un dosage sur membrane, un dispositif pour mettre en AEuvre un tel dosage, et un kit à utiliser pour le dosage.

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