Membrane-bound cytokine compositions and methods of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/11 (2006.01) A61K 35/12 (2006.01) A61K 35/66 (2006.01) A61K 39/00 (2006.01) A61K 39/39 (2006.01) A61K 48/00 (2006.01) C07H 21/04 (2006.01) C07K 14/475 (2006.01) C07K 14/52 (2006.01) C07K 14/535 (2006.01) C07K 14/705 (2006.01) C07K 14/71 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2297747

The present invention provides a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule operatively fused to a heterologous membrane attachment domain. Non-antibody immunomodulatory molecules useful in the invention include immunostimulatory and immunosuppressive molecules such as cytokines. In one embodiment, the invention provides a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule operatively fused to a heterologous membrane attachment domain and, additionally, a disease- associated antigen or immunogenic epitope thereof. Further provided by the invention are methods of modulating an immune response against a disease- associated antigen by administering to an individual a cellular vaccine having a membrane-bound fusion protein that includes a non-antibody immunomodulatory molecule operatively fused to a heterologous membrane attachment domain.

L'invention concerne un vaccin cellulaire à protéine de fusion membranaire comportant une molécule immunorégulatrice non-anticorps fusionnée de manière fonctionnelle avec un domaine de fixation de membrane hétérologue. Ce type de molécule, utile aux fins de l'invention, englobe des molécules à caractère immunostimulateur et immunosuppresseur comme les cytokines. Selon une variante, on décrit un vaccin cellulaire qui comprend une protéine de fusion membranaire renfermant une molécule immunorégulatrice non-anticorps fusionnée de manière fonctionnelle avec un domaine de fixation de membrane hétérologue et, en outre, un antigène associé à une maladie ou un épitope immunogène de cet antigène. L'invention concerne également des procédés permettant la modulation d'une réponse immune face à un antigène associé à une maladie, qui consistent à administrer à un individu un vaccin cellulaire à protéine de fusion membranaire comportant une molécule immunorégulatrice non-anticorps fusionnée de manière fonctionnelle avec un domaine de fixation de membrane hétérologue.

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