C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/542 (2006.01) G01N 33/92 (2006.01)
Patent
CA 2430336
The invention provides membrane molecule indicators, including polypeptides, encoding nucleic acid molecules and cells containing such polypeptides and nucleic acid molecules. The invention membrane molecule indicators are characterized in that fluorescence resonance energy transfer (FRET) between a donor fluorescent domain and an acceptor fluorescent domain indicates a property of the membrane molecule. Also provided are methods of using the invention membrane molecule indicators to determine a property of a membrane molecule, and to identify compounds that modulates a property of a membrane molecule.
L'invention concerne des indicateurs de molécules membranaires, comprenant des polypeptides, codant des molécules et des cellules d'acide nucléique contenant de tels polypeptides et molécules d'acide nucléique. Les indicateurs de molécules membranaires de l'invention sont caractérisés en ce que le transfert d'énergie de résonance de fluorescence (FRET) entre un domaine fluorescent donneur et un domaine fluorescent accepteur indique une propriété de la molécules membranaires. L'invention concerne également des procédés d'utilisation des indicateurs de molécules membranaires de l'invention qui déterminent une propriété d'une molécules membranaires, et qui identifient des composés modulant une propriété d'une molécules membranaires.
Smart & Biggar
The Netherlands Cancer Institute
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