Membrane protein interaction assays

Details Membrane protein interaction assays Membrane protein interaction assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/00 (2006.01) C12Q 1/48 (2006.01) G01N 33/53 (2006.01) G01N 33/554 (2006.01) G01N 33/566 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2361118

In some aspects, the invention provides systems for analysis of protein- protein interactions in which the luminal domain of Ire1p, or homologues thereof, is replaced with the domains of heterologous proteins of interest that then mediate either homo- or heterodimerization of the chimeric fusion proteins to activate a reporter system that may be based on the unfolded protein response pathway (similar to the native activity of Ire1p). -29-

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