Mercury binding polypeptides and nucleotides coding therefor

Details Mercury binding polypeptides and nucleotides coding therefor Mercury binding polypeptides and nucleotides coding therefor

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) C07K 16/44 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) A62D 3/00 (2006.01)

Patent

CA 2182190

Metal binding polypeptides which include an amino acid sequence coding for a variable region of a monoclonal antibody which immunoreacts with a mercury cation and nucleotides which include a nucleic acid sequence coding for the variable region are provided. The invention is also directed to fusion proteins which include a phage coat protein or portion thereof and the monoclonal antibody heavy chain variable region. The invention also provides bacteriophages which include the fusion protein in their coat. In addition, methods for detecting, removing, adding, or neutralizing mercuric cations in biological or inanimate systems through the use of the mercury binding polypeptides are provided.

L'invention concerne des polypeptides de fixation au métal comprenant une séquence d'acides aminés codant pour une région variable d'un anticorps monoclonal qui présente une immunoréactivité avec un cation de mercure et des nucléotides comprenant une séquence d'acides nucléiques codant pour ladite région variable. Elle concerne également des protéines de fusion comprenant une protéine totale ou partielle de coque de phage et la région variable de chaîne lourde de l'anticorps monoclonal. Elle concerne également des bactériophages, dont la coque comprend la protéine de fusion. Elle concerne, de plus, des procédés de détection, de suppression, d'addition ou de neutralisation de cations de mercure dans des systèmes biologiques ou inanimés au moyen desdits polypeptides de fixation au mercure.

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