C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C12P 1/04 (2006.01) C12P 7/40 (2006.01)
Patent
CA 2754470
The present invention provides for novel metabolic pathways leading to acrylate formation in a consolidated bioprocessing system (CBP) where lignocellulosic biomass is efficiently converted to acrylate. In one such metabolic pathway, pyruvate is converted to lactate, which is converted to lactoyol-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. hi another such metabolic pathway, pyruvate is converted to L- .alpha.- alanine, which is converted to L-aspartate, which is converted to .beta.-alanine, which is converted to .beta.-alanyl-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In yet another metabolic pathway, pyruvate is converted to lactate, and then lactate is converted directly to acrylate. In certain aspects, the invention provides for heterologous expression of one or more enzymes in a mesophilic or thermophilic organism, such as Thermoanaerobacterium saccharolyticum or Clostridium thermocellutn, where the one or more enzymes functions within a novel metabolic pathway as described above to convert pyruvate to acrylate via lactate, or via .beta. alanine and acryloyl-CoA.
La présente invention porte sur de nouvelles voies métaboliques conduisant la formation d'acrylate dans un système de biotraitement consolidé (CBP) où une biomasse lignocellulosique est efficacement convertie en acrylate. Dans une telle voie métabolique, le pyruvate est converti en lactate, qui est converti en lactoyl-CoA, qui est convertie en acryloyl-CoA, qui est finalement convertie en acrylate. Dans une autre de ces voies métaboliques, le pyruvate est converti en L-a-alanine, qui est convertie en L-aspartate, qui est converti en ß-alanine, qui est convertie en ß-alanyl-CoA, qui est convertie en acryloyl-CoA, qui est finalement convertie en acrylate. Dans encore une autre voie métabolique, le pyruvate est converti en lactate, puis le lactate est converti directement en acrylate. Sous certains aspects, l'invention concerne l'expression hétérologue d'une ou de plusieurs enzymes dans un organisme mésophile ou thermophile, tel que Thermoanaerobacterium saccharolyticum ou Clostridium thermocellum, la ou les enzymes agissant à l'intérieur d'une nouvelle voie métabolique telle que décrite ci-dessus pour convertir le pyruvate en acrylate par l'intermédiaire du lactate, ou par l'intermédiaire de la ß-alanine et de l'acryloyl-CoA.
Rajgarhia Vineet
Shaw Arthur J. IV
Gowling Lafleur Henderson Llp
Mascoma Corporation
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