Metabolic controlled fermentation procedure for the...

C - Chemistry – Metallurgy – 12 – P

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C12P 17/06 (2006.01) C12P 7/42 (2006.01) C12P 7/62 (2006.01)

Patent

CA 2289553

A method for producing mevinolin by a microorganism in a fermentation process having a seed culture stage and a main fermentation stage, including a) cultivating a microorganism biomass in the seed culture stage to produce an inoculum; b) transferring the inoculum into a fermentation medium in the main fermentation stage; and, c) maintaining steady stage conditions in the main fermentation stage, thereby producing a fermentation broth containing mevinolin. Preferably, the steady state conditions are maintained in the main fermentation stage by one or more of feeding of organic carbon sources; controlling glucose and/or total reducing sugar content; feeding of organic nitrogen sources; controlling pH; controlling foam level; controlling the mass of the fermentation broth by withdrawals and feedings; and, controlling the dissolved oxygen level.

L'invention concerne un procédé de production de mévinoline par un micro-organisme dans un procédé de fermentation comportant une étape de culture de germes et une étape de fermentation principale, qui consiste à: a) mettre en culture une biomasse de micro-organismes à l'étape de culture de germes afin de produire un inoculum; b) transférer l'inoculum dans un milieu de fermentation à l'étape de fermentation principale; et c) maintenir des conditions constantes à l'étape de fermentation principale pour produire ainsi un bouillon de fermentation contenant de la mévinoline. De préférence, on maintient des conditions constantes à l'étape de fermentation principale en mettant en oeuvre une ou plusieurs des actions suivantes: apport de sources de carbone organique; régulation de la teneur en glucose et/ou en sucre réducteur total; apport de sources d'azote organique; régulation du pH; régulation du niveau de mousse; régulation de la masse du bouillon de fermentation par des retraits et des apports; et régulation du taux d'oxygène dissous.

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