Metabolic controlled fermentation process for pseudomonic...

C - Chemistry – Metallurgy – 12 – P

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C12P 17/16 (2006.01) C12N 1/20 (2006.01) C12P 17/18 (2006.01)

Patent

CA 2448547

An improved fermentation process for preparing pseudomonic acid A (mupirocin) is disclosed. The metabolically controlled fermentation process provides culturing a Pseudomonas sp. strain in a submerged medium at a temperature within about 20- 5 30~C. The pH of the fermentation medium is regulated to be at about 5.5-6.0 by feeding the fermentation medium with an assimilable carbon source, a mineral salt, or an acidic/alkali solution. Accordingly, the resulting fermentation broth contains an increased yield of highly purified pseudomonic acid A as the main component. The pseudomonic acid B as an impurity in the fermentation broth is significantly decreased.

L'invention concerne un procédé de fermentation amélioré pour la préparation d'acide A pseudomonique (mupirocine). Ledit procédé de fermentation à régulation métabolique permet de cultiver une souche de Pseudomonas sp. dans un milieu immergé à une température se situant environ entre 20 et 30.ordm.C. Le pH du milieu de fermentation est régulé pour que sa valeur se situe environ entre 5,5 et 6,0; par apport d'une source de carbone assimilable, d'un sel minéral, ou d'une solution acide/alcaline au milieu de fermentation. Par conséquent, le bouillon de fermentation obtenu contient une valeur élevée d'acide A pseudomonique hautement purifié comme composant principal. Les proportions d'acide B pseudomonique présent en tant qu'impureté dans le bouillon de fermentation sont réduites de manière significative.

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