Metabolic engineering for improved xylose utilisation of...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/10 (2006.01) C12N 1/19 (2006.01) C12N 9/04 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2479736

The present invention relates to a method for preparing an ethanol producing, xylose utilizing strain of Saccharomyces cerevisiae comprising genes for overexpression of xylose reductase, xylitol dehydrogenase and xylulokinase, wherein in addition to said genes, the genes for production of phosphoacetyltransferase, and acetaldehyde dehydrogenase are introduced and optionally overexpressed.

La présente invention concerne un procédé de préparation d'une souche de Saccharomyces cerevisiae utilisant le xylose et produisant de l'éthanol qui comprend des gènes servant à la surexpression de la xylose réductase, de la xylose réductase, et de la xylitol déshydrogénase. Dans ce procédé, outre l'addition desdits gènes, les gènes produisant la phosphoacétyltransférase et l'acétaldéhyde déshydrogénase sont introduits et éventuellement surexprimés.

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