Metabolic engineering of arabinose- fermenting yeast cells

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/18 (2006.01) C12P 7/08 (2006.01)

Patent

CA 2664646

The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and/or convert L-arabinose into L-ribulose, and/or xylulose 5-phosphate and/or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.

L'invention concerne des séquences de nucléotides exprimant une cellule eucaryote codant pour les enzymes ara A, ara B et ara D. Grâce à l'invention, l'expression de ces séquences de nucléotides confère à la cellule la capacité d'utiliser la L-arabinose et/ou de convertir la L-arabinose en L-ribulose, et/ou en xylulose 5-phosphate et/ou en un produit de fermentation souhaité tel que l'éthanol. En option, la cellule eucaryote est également capable de convertir le xylose en éthanol.

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