Metabolically engineered bacterial strains having enhanced...

Details Metabolically engineered bacterial strains having enhanced... Metabolically engineered bacterial strains having enhanced...

C - Chemistry, Metallurgy

12

P

C12P 1/00 (2006.01) C07H 21/04 (2006.01) C12N 9/04 (2006.01) C12P 1/04 (2006.01) C12P 7/00 (2006.01) C12P 7/26 (2006.01)

Patent

CA 2533936

The present invention relates to a method of altering bacterial host cells to accumulate 2-keto-D-gluconic acid (2-KDG) by inactivating an endogenous membrane bound 2-keto-D-gluconate dehydrogenase (2-KDGDH), which prior to inactivation catalyzed the conversion of 2-KDG to 2,5-diketogluconate (2,5- DKG).

L'invention concerne un procédé pour modifier des cellules hôtes bactériennes de manière à accumuler de l'acide 2-céto-D-gluconique (2-KDG) par inactivation d'une membrane endogène liée à une déshydrogénase 2-céto-D-gluconate (2-KDGDH), laquelle a catalysé la conversion avant l'inactivation du 2-KDG en 2,5-dicétogluconate (2,5-DKG).

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