Metabolically engineered cells for the production of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12P 7/22 (2006.01)

Patent

CA 2598414

A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4- hydroxylase (C4H) produces 4-coumaric acid from said trans- cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4- coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4- coumaroyl CoA, or in which L-phenylalanine- or tyrosine- ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4- coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.

Microorganisme recombiné produisant du resvératrol par une voie dans laquelle la phénylalanine ammonia-lyase (PAL) produit de l'acide trans-cinnamique à partir de phénylalanine, la cinnamate 4-hydroxylase (C4H) produit de l'acide 4-coumarique à partir dudit acide trans-cinnamique, la 4-coumarate-CoA ligase (4CL) produit de la 4-coumaroyl-CoA à partir dudit acide 4-coumarique et la resvératrol synthétase (VST) produit ledit resvératrol à partir de ladite 4-coumaroyl-CoA ou bien dans laquelle la L-phénylalanine ou tyrosine ammonia-lyase (PAL/TAL) produit de l'acide 4-coumarique, la 4-coumarate-CoA ligase (4CL) produit de la 4-coumaroyl-CoA à partir dudit acide 4-coumarique et la resvératrol synthétase (VST) produit ledit resvératrol à partir de ladite 4-coumaroyl-CoA. Le microorganisme peut être une levure, un champignon ou une bactérie dont Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger ou Aspergillus oryzae.

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