Metachromatic dye sorption means for differential...

G - Physics – 01 – N

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150/13

G01N 1/30 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1191079

ABSTRACT OF THE DISCLOSURE In the diagnosis and prognosis of various diseases as well as medical researches in relation thereto studies leading to diferentiation, identification, enumeration and following the variations in human blood, leukocytes play a vital role. Heretofore, accurate detailed lengthy and arduous procedures were essential to such ends, if available. In a parent case, of which this is a continuation-in-part, it was found selective use of certain basic quaternary, metachromatic dye staining by supravital techniques made possible instantaneous optical differentiation of the five major individual leukocyte species. Here further research has revealed that with but one of the species of the dye class above one can by the same techniques make the foregoing determinations with regard to specific leukocytes including promyelocytes, myelocytes, metamyelocytes, band cells, T-cells and B-cells as well as the neutrophils, eosinophils, basophils and monocytes of the parent disclosure.

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