C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12N 1/14 (2006.01) C12N 1/20 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)
Patent
CA 2383460
The present invention is directed generally to metal binding compounds which may be added to cell culture media to replace factors required for cultivation of the cells (e.g. transferrin) which are of animal or human origin. More specifically, the invention is directed to metal binding compounds or complexes thereof comprising one or more transition element cations (such as ferrous or ferric ions), which are added to cell and tissue culture medium compositions. The metal binding compounds may be added to the media alone or may be first complexed with a transition metal ion. The invention is also directed to methods of use of such compositions, including, for example, methods for the cultivation of eukaryotic cells, particularly animal cells, in vitro. The invention also relates to compositions comprising such culture media and one or more cells, and to kits comprising one or more of the above- described compositions. The compositions of the present invention obviate the need for naturally derived metal-binding proteins, such as transferrin and ceruloplasmin, which may contain blood-borne pathogens.
L'invention concerne en général des composés de liaison métalliques pouvant être ajoutés à des milieux de culture cellulaire afin de remplacer des facteur requis pour la culture de cellules (par exemple, la transferrine) d'origine animale ou humaine. L'invention concerne, plus particulièrement, des composés de liaison métalliques ou des complexes de ceux-ci, comprenant au moins des cations d'élément de transition (tels que des ions ferreux ou ferriques), qui sont ajoutés aux compositions de milieu de culture cellulaire et tissulaire. Les composés de liaison métalliques peuvent être ajoutés au milieu seul, ou peuvent d'abord être complexés avec un ion de métal de transition. L'invention concerne également des procédés d'utilisation de ces compositions, notamment, par exemple, des procédés de culture de cellules eukaryotiques, en particulier des cellules animales in vitro. L'invention concerne, en outre, des compositions comprenant ce milieu de culture et au moins une cellule, et des kits comprenant au moins l'une des compositions précitées. Les compositions de l'invention rendent inutile le recours à des protéines de liaison de métal dérivées naturellement, telles que des protéines extraites de la transferrine et la ceruloplasmine, qui peuvent comprendre des pathogènes à diffusion hématogène.
Battista Paul
Epstein David A.
Gruber Dale
Judd David
Invitrogen Corporation
Life Technologies Corporation
Mbm Intellectual Property Law Llp
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