Metal binding proteins, recombinant host cells and methods

Details Metal binding proteins, recombinant host cells and methods Metal binding proteins, recombinant host cells and methods

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/00 (2006.01) C07K 14/25 (2006.01) C07K 14/47 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2424295

The present disclosure provides artificial heave metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metal taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

L'INVENTION CONCERNE DES PROTEINES ARTIFICIELLES CAPABLES DE LIAISON AVEC LES METAUX LOURDS APPELEES <= CHELONS >= PAR LES INVENTEURS. CES CHELONS FIXENT LES IONS DE CADMIUM ET/OU DE MERCURE AVEC UNE AFFINITE RELATIVEMENT ELEVEE. PAR AILLEURS, L'INVENTION CONCERNE DES SEQUENCES CODANTES, DES MOLECULES D'ADN RECOMBINEES ET DES CELLULES HOTES RECOMBINEES CONTENANT CES MOLECULES D'ADN RECOMBINEES POUR L'EXPRESSION DES PROTEINES CHELONS. DANS LES CELLULES HOTES RECOMBINEES OU LES PLANTES TRANSGENIQUES, ON PEUT UTILISER LES CHELONS POUR FIXER LES METAUX LOURDS EXTRAITS DU SOL CONTAMINE, DES EAUX SOUTERRAINES OU DE L'EAU D'IRRIGATION, ET POUR CONCENTRER ET SEQUESTRER CES IONS. LES BACTERIES ENTERIQUES RECOMBINEES PEUVENT ETRE UTILISEES DANS LE TRACTUS INTESTINAL DES ANIMAUX OU DES HUMAINS EXPOSES A DES IONS DE METAL TOXIQUE TEL QUE LE MERCURE ET/OU LE CADMIUM; LE CHELON EXPRIME PAR RECOMBINAISON ETANT CHOISI EN FONCTION DE L'ION A ELIMINER. DANS UNE VARIANTE, ON PEUT IMMOBILISER LES CHELONS SUR DES SUPPORTS SOLIDES POUR FIXER ET CONCENTRER LES METAUX LOURDS DANS UN MILIEU AQUEUX CONTAMINE CONTENANT DES LIQUIDES BIOLOGIQUES.

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