Metapneumovirus strains and their use in vaccine...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/45 (2006.01) A61K 35/76 (2006.01) A61K 39/155 (2006.01) A61P 31/14 (2006.01) C07K 14/11 (2006.01) C07K 14/115 (2006.01) C07K 14/135 (2006.01) C07K 16/10 (2006.01) C12N 7/00 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2477234

The present invention provides an isolated mammalian negative strand RNA virus, metapneumovirus (MPV), within the sub-family Pneumoviridae, of the family Paramyxoviridae. The invention also provides isolated mammalian negative strand RNA viruses identifiable as phylogenetically corresponding or relating to the genus Metapneumovirus and components thereof. In particular the invention provides a mammalian MPV, subgroups and variants thereof. The invention relates to genomic nucleotide sequences of different isolates of mammalian metapneumoviruses, in particular human metapneumoviruses. The invention relates to the use of the sequence information of different isolates of mammalian metapneumoviruses for diagnostic and therapeutic methods. The present invention relates to nucleotide sequences encoding the genome of a metapneumovirus or a portion thereof, including both mammalian and avian metapneumovirus. The invention further encompasses chimeric or recombinant viruses encoded by said neucleotide sequences. The invention also relates to chimeric and recombinant mammalian MPV that comprise one or more non-native or heterologous sequences. The invention further relates to vaccine formulations comprising mammalian or avian metapneumovirus, including recombinant and chimeric forms of said viruses. The vaccine preparation of the invention encompass multivalent vaccines, including bivalent and trivalent vaccine preparations.

Cette invention concerne un virus à ARN de polarité négative mammifère isolé, le métapneumovirus (MPV) appartenant, dans la sous-famille des Pneumoviridae, à la famille des Paramyxoviridae. Cette invention concerne également des virus à ARN de polarité négative mammifères isolés pouvant être identifiés comme correspondant phylogénétiquement ou étant associé au genre Métapneumovirus et à ses composants et concerne en particulier un métapneumovirus mammifère, ainsi que des sous-groupes et des variantes de celui-ci. Cette invention porte également sur des séquences nucléotidiques génomiques de différents isolats de métapneumovirus mammifères, en particulier de métapneumovirus humains ; sur l'utilisation des informations de séquence de différents isolats de métapneumovirus mammifères dans des méthodes diagnostiques et thérapeutiques ; sur des séquences nucléotidiques codant le génome d'un métapneumovirus ou une partie de celui-ci, y compris le métapneumovirus mammifère et aviaire ; sur des virus chimériques et recombinants codés par ces séquences nucléotidiques ; sur un métapneumovirus mammifère chimérique et recombinant qui comprend une ou plusieurs séquences non natives ou hétérologues ; ainsi que sur des préparations vaccinales comprenant le métapneumovirus mammifère ou aviaire, y compris des formes recombinantes et chimériques de ces virus. Les préparations vaccinales de la présente invention comprennent des vaccins multivalents, y compris des préparations vaccinales bivalentes et trivalentes.

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