C - Chemistry – Metallurgy – 07 – C
Patent
C - Chemistry, Metallurgy
07
C
167/43, 530/15.1
C07C 225/16 (2006.01) C07K 14/435 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/94 (2006.01)
Patent
CA 2008277
ABSTRACT A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.
Dubler Robert E.
Thacker Susan A.
Walling John A.
Wang Nai-Yi
Abbott Laboratories
Goudreau Gage Dubuc
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