Method and a kit for the assay of antibodies to soluable...

G - Physics – 01 – N

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G01N 33/538 (2006.01)

Patent

CA 1177391

ABSTRACT OF THE DISCLOSURE The invention relates to a method for the assay of anti- bodies to soluble antigens in an aqueous sample, in particular in body fluids, such as blood serum or blood plasma, by contact- ing said sample with an antigen in vitro, wherein antibodies, if present, are bound by said antigens. According to the invention an antigen is used that is modified with a recognizable group, and said modified antigen is soluble in the test sample. The interaction between antigen and antibody takes place in homoge- neous solution. The invention furthermore relates to a kit for the assay and detection of antigen-specific antibodies. The method of the present invention overcomes the prior disadvantage of the preparation, storage and dispensation of a large number of solid-phase-coupled antigens and provides reaction condi- tions which are essentially similar to the physiological condi- tions, where the antigen is in solution when it reacts with the antibody.

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