Method and apparatus for a microscope image selector

G - Physics – 01 – J

Patent

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Details

G01J 3/00 (2006.01) G01J 3/28 (2006.01) G01J 3/40 (2006.01) G01J 3/42 (2006.01) G01J 3/44 (2006.01) G01N 21/25 (2006.01)

Patent

CA 2596077

The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample (100). In one embodiment, the apparatus includes an image selector (108) having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector (108) adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret (102) housing a second plurality of components, the microscope turret (102) adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret (102) adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

L'invention porte d'une manière générale sur un appareil d'imagerie multimode permettant d'obtenir simultanément plusieurs images spectrales d'un échantillon se distinguant les unes des autres par leur longueur d'onde. Dans une exécution, l'appareil comporte: un sélecteur d'images muni d'un rotateur comportant plusieurs composants optiques et conçu pour: recevoir des photons éclairants d'une première longueur d'onde et les diriger sur l'échantillon; recevoir les photons ayant interagi avec l'échantillon et les diriger sélectivement vers l'une des sources de détection. L'appareil comporte également une tourelle de microscope comportant un deuxième ensemble de composants optiques et conçue pour: recevoir des photons éclairants d'une deuxième longueur d'onde et les diriger sur l'échantillon; recevoir les photons ayant interagi avec l'échantillon et les diriger sélectivement vers l'une des sources de détection. La quasi totalité des photons ayant interagi avec l'échantillon, et dirigés sélectivement sur l'une des sources de détection, forment simultanément plusieurs images spectrales de l'échantillon, distinctes les unes des autres par leur longueur d'onde.

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