Method and apparatus for analysis of particles in a liquid...

G - Physics – 06 – T

Patent

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Details

G06T 7/00 (2006.01) G01N 15/06 (2006.01) G01N 21/84 (2006.01) G01N 33/487 (2006.01) G01N 33/49 (2006.01) G01N 33/493 (2006.01)

Patent

CA 2714903

The invention relates to methods for analysis of particles in a liquid sample, the sample being retained in a sample retaining device, the method comprising: acquiring, by means of an image acquiring device, a plurality of images of said sample at different focus planes within the sample retaining device; analysing said images, by means of an image analyser, for identifying which, if any, of the particles of the sample are imaged in focus in each of the images, and analysing those particles which have been identified as being imaged in focus; wherein said plurality of images are acquired at different, essentially parallel, focus planes, which planes are separated from each other by a distance, said distance being less than 10 micrometers. The invention also relates to apparatuses adapted to be used for the inventive methods.

L'invention concerne des procédés d'analyse de particules dans un échantillon liquide, l'échantillon étant conservé dans un dispositif qui contient des échantillons, le procédé comprenant : l'acquisition, à l'aide d'un dispositif d'acquisition d'images, d'une pluralité d'images dudit échantillon à différents plans de mise au point au sein du dispositif qui contient l'échantillon; l'analyse desdites images, à l'aide d'un analyseur d'images, afin d'identifier, le cas échéant, les particules de l'échantillon qui font l'objet d'une mise au point sur chacune des images, et l'analyse des particules qui ont été identifiées comme faisant l'objet de la mise au point; ladite pluralité d'images étant acquise à différents plans de mise au point, essentiellement parallèles, lesdits plans étant séparés les uns des autres par une certaine distance, ladite distance étant inférieure à 10 micromètres. L'invention concerne également des appareils conçus pour être utilisés pour les procédés de l'invention.

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