Method and apparatus for assay for multiple analytes

G - Physics – 01 – N

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G01N 21/77 (2006.01) G01N 21/47 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2403427

A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate (12) upon which is arranged a multiplicity of recognition elements (14, 16, 18), such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a characteristic diffraction pattern in the assay. The patterns may or may not be interpenetrating on the substrate surface. The method of detecting multiple analytes includes contacting the medium of analytes with the patterned substrate, illuminating the substrate by a light source, and detecting any resultant diffraction image. The pattern of diffraction and the intensity of the diffracted signal provides information about the existence of specific analytes and their quantification.

Procédé et appareil pour un dosage destiné aux analytes multiples. Le procédé utilise un élément de détection qui comprend un substrat sur lequel sont disposés plusieurs éléments de reconnaissance, de manière à ce que chaque élément soit disposé selon un motif prédéterminé. Chaque motif est unique en ce qu'il permet un motif de diffraction caractéristique dans le dosage. Les motifs peuvent éventuellement s'interpénétrer avec la surface du substrat. Le procédé de détection des analytes multiples consiste à mettre en contact le milieu des analytes avec le substrat comportant des motifs, éclairer le substrat avec une source lumineuse et détecter les éventuelles images de diffraction. Le motif de diffraction et l'intensité du signal de diffraction fournissent des informations sur l'existence d'analytes déterminés et permettent leur quantification.

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