Method and apparatus for automated identification and...

Details Method and apparatus for automated identification and... Method and apparatus for automated identification and...

G - Physics

01

N

73/51.5

G01N 21/64 (2006.01) G01N 15/14 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1133274

ORI-23 Method and Apparatus for Automated Identification and Enumeration of Specified Blood Cell Subclasses Abstract of the Disclosure Specified subclasses of blood cells, such as of lympho- cytes, are automatically identified based on utilization of antigenic determinants on the cell surface, their reactivity with antibodies which fluoresce under known circumstances, and utilization of principles of flow cytometry. A blood sample is first incubated with a reagent including antibodies to the lymphocyte subclass to be identified, the antibodies being directly or indirectly made fluorescently responsive to particular light (e. g. argon ion laser). The sample is illuminated, a cell at a time, by such focused coherent light, and forward light scatter, right angle light scatter, and fluorescence are detected and used to identify and enumerate cells of the specified subclass.

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