Method and apparatus for automated reprocessing of tissue...

G - Physics – 01 – N

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G01N 1/30 (2006.01) G01N 1/31 (2006.01)

Patent

CA 2355114

A method and apparatus of automatically reprocessing a specimen for microscopic exam-ination is disclosed. Processing of a specimen for microscopic examination involves fixation of the specimen and preparation of the embedded specimen from the fixed specimen. There are instances where, once a specimen has been pro-cessed, it is necessary to reprocess the specimen due to contamination of reagents during process-ing or inadequate fixation. The system automati-cally reprocesses a specimen by removing resid-ual embedding material from the specimen with a clearing agent, removing the clearing agent with a dehydrating agent, and removing the dehydrat-ing agent with an aqueous fluid.

L'invention concerne un procédé et un dispositif de retraitement automatique d'un spécimen, aux fins d'examen au microscope. Le traitement d'un spécimen, aux fins d'examen au microscope, consiste à fixer celui-ci, et à préparer un spécimen incorporé à partir du spécimen fixé. Il existe des cas dans lesquels, une fois que le spécimen a été traité, il est nécessaire de le retraiter, par suite de la contamination provoquée par des réactifs lors du traitement, ou par suite d'une fixation inadéquate. Ce système retraite automatiquement un spécimen en enlevant le matériau résiduel d'incorporation, à partir de l'échantillon, au moyen d'un agent de clarification et d'un agent de déshydratation, et en enlevant l'agent de déshydratation au moyen d'un fluide aqueux.

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