Method and apparatus for automatically detecting...

G - Physics – 01 – N

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G01N 33/483 (2006.01) G01N 15/14 (2006.01) G01N 33/50 (2006.01) G06K 9/00 (2006.01) G06K 9/20 (2006.01) G06K 9/62 (2006.01) G06K 9/80 (2006.01)

Patent

CA 2253850

A method for detecting malignancy-associated changes. A sample of cells is obtained and stained to identify the nuclear DNA material. The sample is imaged with a digital microscope. Objects of interest are identified in the sample of cells based on the intensity of the pixels that comprise the object versus the average intensity of all pixels in the slide image. An exact edge is located for each object and variations in the illumination intensity of the microscope are compensated for. A computer system calculates feature values for each object and, based on the value of the features, a determination is made whether the cell exhibits malignancy-associated changes or not.

L'invention porte sur un procédé de détection de changements associés à une malignité. On prélève un échantillon de cellules et on le colore pour identifier le matériel d'ADN nucléaire. L'échantillon est mis en image à l'aide d'un microscope numérique. Les objets d'intérêt sont identifiés dans l'échantillon de cellules sur la base de l'intensité des pixels que comporte l'objet par rapport à l'intensité moyenne de l'ensemble des pixels de l'image de la lame. La lisière exacte est localisée pour chaque objet et les variations d'intensité de l'éclairage du microscope sont compensées. Un système d'ordinateur calcule des valeurs caractéristiques pour chacun des objets, et sur la base de la valeur de ces caractéristiques on détermine si la cellule présente ou non des changements associés à une malignité.

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