Method and apparatus for avalanche-mediated transfer of...

Details Method and apparatus for avalanche-mediated transfer of... Method and apparatus for avalanche-mediated transfer of...

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01)

Patent

CA 2643078

The present invention provides a method and apparatus for transferring an agent into a cell. The method includes the steps of providing an agent outside of a cell and generating a vapor bubble and a plasma discharge between an avalanche electrode and a conductive fluid surrounding the cell. The vapor bubble and plasma discharge generate a mechanical stress wave and an electric field, respectively. The combination of this mechanical stress wave and electric field results in permeabilization of the cell, which in turn results in transfer of the agent into the cell.

La présente invention concerne un procédé et un appareil permettant de transférer un agent dans une cellule. Le procédé comprend les étapes consistant à préparer un agent en dehors d'une cellule et à générer une bulle de vapeur et une décharge de plasma entre une électrode à avalanche et un fluide conducteur entourant la cellule. La bulle de vapeur et la décharge de plasma génèrent respectivement une onde de contrainte mécanique et un champ électrique. La combinaison de cette onde de contrainte mécanique et de ce champ électrique a pour effet de rendre la cellule perméable, ce qui permet alors de transférer l'agent dans la cellule.

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