Method and apparatus for chromosome profiling

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2595897

A method and apparatus for generating an Interphase chromosome profile. The method comprises obtaining a sample containing cells having chromosomes for profiling; obtaining species specific DNA probes, wherein the DNA probes are capable of marking at least one chromosome at substantially equidistant locations on said chromosomes; hybridizing the sample with the DNA probes; using a plurality of enzymes to produce differential color bands on the chromosome for colorimetric analysis of the sample; and using visual analysis for determining the profile of the chromosome.

Procédé et appareil de génération d'un profil de chromosome Interphase. Le procédé consiste à obtenir un échantillon contenant des cellules présentant des chromosomes à profiler; à obtenir des sondes ADN spécifiques aux espèces, lesquelles sondes étant aptes à marquer au moins un chromosome à des emplacements sensiblement équidistants sur ledit chromosome; à hybrider l'échantillon avec les sondes ADN; à utiliser une pluralité d'enzymes pour produire des bandes de couleurs différentielles sur le chromosome en vue d'une analyse colorimétrique de l'échantillon; et à utiliser une analyse visuelle pour déterminer le profil du chromosome.

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