Method and apparatus for cryopreparation, dry stabilization...

Details Method and apparatus for cryopreparation, dry stabilization... Method and apparatus for cryopreparation, dry stabilization...

C - Chemistry, Metallurgy

12

N

C12N 1/04 (2006.01) A01N 1/00 (2006.01) A01N 1/02 (2006.01) A61K 35/18 (2006.01) A61K 39/13 (2006.01) C12N 5/02 (2006.01) F25C 1/14 (2006.01) F25D 31/00 (2006.01) F26B 5/06 (2006.01)

Patent

CA 2051092

This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.

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