Method and apparatus for desorption and ionization of analytes

Details Method and apparatus for desorption and ionization of analytes Method and apparatus for desorption and ionization of analytes

H - Electricity

01

J

H01J 49/02 (2006.01) G01N 27/62 (2006.01) G01N 30/72 (2006.01) H01J 49/26 (2006.01)

Patent

CA 2512290

This invention relates generally to methods and apparatus for desorption and ionization of analytes for the purpose of subsequent scientific analysis by such methods, for example, as mass spectrometry or biosensors. More specifically, this invention relates to the field of mass spectrometry, especially to the type of matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry used to analyze macromolecules, such as proteins or biomolecules. Most specifically, this invention relates to the sample probe geometry, sample probe composition, and sample probe surface chemistries that enable the selective capture and~ desorption of analytes, including intact macromolecules, directly from the probe surface into the gas (vapor) phase without added chemical matrix.

L'invention concerne des procédés et un dispositif de désorption et d'ionisation d'analytes, dans la perspective d'autres analyses scientifiques effectuées en faisant par exemple appel à la spectrométrie de masse ou à des détecteurs biologiques. Cette invention concerne plus spécifiquement le domaine de la spectrométrie de masse, notamment le type de spectrométrie de masse à temps de vol par désorption/ionisation laser assistée par matrice, utilisée pour analyser des macromolécules comme des protéines ou des biomolécules. L'invention concerne plus spécifiquement la géométrie, la composition et les propriétés chimiques de surface de la sonde d'échantillonnage, qui permettent la capture sélective et la désorption d'analytes, y compris de macromolécules intactes, directement depuis la surface de la sonde pour les faire passer en phase gazeuse (vapeur) sans adjonction de matrice chimique.

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