Method and apparatus for detecting fluorescence emitted by...

G - Physics – 01 – N

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G01N 21/64 (2006.01) B81B 1/00 (2006.01) G02B 21/00 (2006.01)

Patent

CA 2703716

A method of detecting a fluorescence signal emitted by fluorophores bound to particles confined in a particle trap, includes an objective lens having a focal plane, which is normally the focal plane for incident collimated light. The particle trap is typically located in the focal plane, and a beam of excitation light is directed via the objective lens onto the confined particles in the trap. The excitation light is in the form of a divergent beam coming to focus at a plane displaced from the focal plane. The divergent beam has a spot diameter at the focal plane determined by the divergence of the beam. The fluorescent light emitted by the fluorophores is detected with a confocal detector.

La présente invention concerne un procédé de détection d'un signal de fluorescence émis par des fluorophores liés à des particules confinées dans un piège à particules. Ledit procédé fait appel à un objectif possédant un plan focal, qui est normalement le plan focal de la lumière collimatée incidente. Le piège à particules est généralement situé dans le plan focal et un faisceau de lumière excitatrice est dirigé, via l'objectif, sur les particules confinées dans le piège. La lumière excitatrice se présente sous la forme d'un faisceau divergent venant se focaliser sur un plan décalé par rapport au plan focal. Le diamètre du faisceau divergent au niveau du plan focal est déterminé par la divergence du faisceau. La lumière fluorescente émise par les fluorophores est détectée au moyen d'un détecteur confocal.

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