Method and apparatus for detecting pharmaceuticals in a sample

Details Method and apparatus for detecting pharmaceuticals in a sample Method and apparatus for detecting pharmaceuticals in a sample

G - Physics

01

N

G01N 21/64 (2006.01)

Patent

CA 2758063

A method and apparatus for measuring a vitamin K antagonizing anticoagulant present in a sample (116), arranged to: irradiate (304) the sample (116) with light from a light source (114) for exciting the anticoagulant through its absorption of the light, the excitation of the sample (116) resulting in a fluorescent emission from the sample (116); measure (306) the fluorescent emission from the sample (116); determine (308) a fluorescence lifetime (T1) of the fluorescent emission of the sample (116); determine (310) an intensity (A1) of the fluorescent emission at the fluorescence lifetime (T1); and determine (312) a amount (c) of the anticoagulant, as a function of the intensity (A1) of the fluorescent emission at the fluorescence lifetime (T1).

La présente invention concerne un procédé et un appareil permettant de mesurer un anticoagulant antagonisant la vitamine K présent dans un échantillon (116), lequel appareil est conçu pour exposer (304) l'échantillon à une lumière provenant d'une source lumineuse (114) afin d'exciter l'anticoagulant grâce à l'absorption de la lumière, l'excitation de l'échantillon (116) donnant lieu à une émission fluorescente depuis l'échantillon (116); pour mesurer (306) l'émission fluorescente émanant de l'échantillon (116); pour déterminer (308) une durée de vie (T1) de la fluorescence de l'émission fluorescente de l'échantillon; pour déterminer (310) une intensité (A1) de l'émission fluorescente sur la durée de vie (T1) de la fluorescence; et pour déterminer (312) une quantité de l'anticoagulant en fonction de l'intensité (A1) de l'émission fluorescente sur la durée de vie (T1) de la fluorescence.

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