Method and apparatus for detection of microscopic pathogens

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2395318

Detection apparatus for use in the detection of the presence of a selected pathogen in a sample are disclosed. Such apparatus includes: a substrate with a detection region on a surface thereof, the detection region having microstructures including grooves formed therein that will align liquid crystal material in contact therewith, the width and depth of the grooves being in the range of 10 µm or less; a blocking layer on the surface of the detection region of the substrate that does not disrupt the alignment of liquid crystal material in contact therewith, the blocking layer blocking nonspecific adsorption of pathogens to the surface; and a binding agent on the surface of the detection region of the substrate, the binding agent specifically binding the selected pathogen.

La présente invention concerne un appareil de détection utilisé pour détecter la présence d'un pathogène choisi dans un prélèvement. L'appareil de l'invention comprend : un substrat dont la surface comporte une région de détection, ladite région de détection étant munie de microstructures comprenant des rainures formées dans la surface qui permettent l'alignement d'une matière à cristaux liquides entrant en contact avec les rainures, les rainures possédant une largeur et une profondeur inférieures ou égales à 10 mu m ; une couche de blocage sur la surface de la région de détection du substrat, qui n'entrave pas l'alignement de la matière à cristaux liquides en contact avec la région de détection, mais qui bloque l'adsorption non spécifique des pathogènes sur la surface ; et un agent de liaison sur la surface de la région de détection du substrat, l'agent de liaison liant spécifiquement le pathogène choisi.

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