Method and apparatus for detection of unsuitable conditions...

G - Physics – 01 – N

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G01N 37/00 (2006.01) G01N 15/14 (2006.01) G01N 21/95 (2006.01) G01N 33/483 (2006.01) G01N 35/00 (2006.01) G02B 21/36 (2006.01) G06K 9/00 (2006.01)

Patent

CA 2200454

A method and apparatus for determining whether a slide is suitable for processing. A suite of suitability tests (16) are performed by an automated microscope system (511). The tests (16) include magnification error flags, staining flags, main optical density flags, including detected intermediate cell nuclei, rings around detected intermediate cell nuclei, average texture measure of detected intermediate cell nuclei, average contrast to detected intermediate cell nuclei to cytoplasm, standard deviation of detected intermediate cell nuclei optical densities, detected intermediate cell ratios, average stripe area, measure of a saturated magnification of the image, measure of a grossly saturated magnification, and the percentage of images focused properly on a first try, including images never focused properly. The automated microscope (511) quantifies the measurements in a reliable and repeatable way.

La présente invention concerne un procédé pour déterminer si une lame de microscope est apte au traitement. Une suite de tests d'aptitude (16) est exécutée au moyen d'un système de microscope (511). Ces tests (16) permettent de déterminer les erreurs de grossissement, la coloration, la densité optique principale. Ces informations concernent notamment les noyaux de cellules intermédiaires détectées, la mesure de texture moyenne des noyaux de cellules intermédiaires détectées, la mesure du contraste moyen entre les noyaux des cellules intermédiaires détectées et le cytoplasme, l'écart standard entre densités optiques des noyaux des cellules intermédiaires détectées, la mesure du niveau de saturation par grossissement de l'image, la mesure du niveau de saturation en grossissement grossier, et le pourcentage d'images correctement mises au point du premier coup ainsi que le pourcentage d'images n'ayant jamais été mises au point correctement. Ce microscope automatisé (511) permet une quantification fiable et fidèle des mesures.

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