Method and apparatus for determination of an analyte and...

G - Physics – 01 – N

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73/124, 354/22,

G01N 21/31 (2006.01) G01D 18/00 (2006.01) G01N 21/27 (2006.01) G01N 33/72 (2006.01)

Patent

CA 1227658

-0- METHOD AND APPARATUS FOR DETERMINATION OF AN ANALYTE AND METHOD OF CALIBRATING SUCH APPARATUS Abstract of the Disclosure A method and apparatus are described for the determination of an analyte in an aqueous liquid while eliminating the biasing effect of unknown interferents which are preformed or formed in situ during the determination. The method comprises physically contacting a sample of the liquid with an interactive composition for the analyte; measuring the spectrophotometric responses generated by such contact at a primary wavelength .lambda.1 and one or more secondary wavelengths .lambda.2, .lambda.3,???.lambda.n; and determining analyte concentration or activity using the equation: C = a0+a1[A1+.alpha.1A2+???+.alpha.n-1An] wherein C is analyte concentration or activity, A1,A2,???An are the spectrophotometric responses observed at .lambda.1,.lambda.2,???.lambda.n, respectively; and a0,a1, and .alpha.1,.alpha.2,???.alpha.n-1 are constants determined according to, a calibrating method, also described herein. Such calibrating method is an empirical means for determining and recording in a chemical analyzer the a0,a1, and .alpha.1,.alpha.2,???.alpha.n-1 constants essential for making the analyte determination.

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