Method and apparatus for determining a contact area between...

Details Method and apparatus for determining a contact area between... Method and apparatus for determining a contact area between...

G - Physics

01

N

G01N 3/42 (2006.01) C12M 1/34 (2006.01) G01B 11/28 (2006.01) G01N 33/483 (2006.01)

Patent

CA 2267454

An optical probe (20) that is transparent contacts a surface (12) of a specimen such that an area of contact is formed. The probe's contact surface (P) has known geometry. By viewing the area of contact through the probe, the area of contact can be determined. The probe may be positioned between the specimen (10) and the objective of a microscope. The microscope and the probe provide a nondestructive and noninvasive instrument to measure the turgor pressure in a cellular specimen. The area of contact between the probe (20) and a cell is a function of the cell's internal pressure. Application of a known force between probe and cell in combination with knowledge of the probe's geometry permits the use of the contact area to provide a measurement of turgor pressure.

L'invention concerne une sonde optique (20) transparente qui est en contact avec une surface (12) d'un échantillon de telle sorte qu'une zone de contact se forme. La surface de contact (P) de la sonde présente une géométrie connue. On peut déterminer la zone de contact en l'observant à travers la sonde. La sonde peut être placée entre l'échantillon (10) et l'objectif d'un microscope. Le microscope et la sonde constituent un moyen non destructeur et non effractif de mesure de la pression hydrostatique intracellulaire dans un échantillon de cellule. La zone de contact entre la sonde (20) et une cellule dépend de la pression interne de la cellule. L'application d'une force connue entre une sonde et une cellule combinée à la connaissance de la géométrie de la sonde permet d'utiliser la zone de contact pour effectuer une mesure de pression hydrostatique intracellulaire.

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