Method and apparatus for determining red blood cell indices...

G - Physics – 01 – N

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G01N 33/49 (2006.01)

Patent

CA 2718995

Method and apparatus for determining the concentration of haemoglobin within red blood cells. An undiluted, whole blood sample is deposited into a transparent analysis chamber (10) whose height is such that the red blood cells are in contact with both surfaces (14, 18). Then the optical density of a portion of the red blood cells in contact with both' surf aces is measured. The haemoglobin concentration of the red blood cell is calculated from the optical density and the known distinction coefficient of haemoglobin. This is repeated with other cells until an average haemoglobin concentration can be determined with confidence. Dependent claims include use of zwitterionic isovolumetric sphering agent make the measurement independent of a geometrical form factor.

La présente invention concerne un procédé pour la détermination dindices dérythrocytes comprenant le volume et la teneur et la concentration en hémoglobine pour des érythrocytes individuels, ainsi que des statistiques de population dérythrocytes comprenant le nombre total dérythrocytes présents dans léchantillon, et des valeurs moyennes pour chacun des indices susmentionnés dans un échantillon sanguin sensiblement non dilué.

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