Method and apparatus for determining the count per unit...

G - Physics – 01 – N

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354/22, 73/59, 3

G01N 15/14 (2006.01)

Patent

CA 1272805

ABSTRACT OF THE INVENTION A method and apparatus for accurately determining the count per unit volume of particles, such as red blood cells, suspended in a fluid containing at least one undesirable interference particle, such as air bubbles. A predetermined volume of fluid containing the first and second particles is passed through the a flow cytometer which produces a forward light scattering pattern that is detected and measured. The volume and index refraction of each of the first and second particles is determined. The first and second particles are discriminated in accordance with their respective indices of refraction and the total count and volume of particles is corrected to provide the count per unit volume of red blood cells.

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