Method and apparatus for filtration of bioreactor...

B - Operations – Transporting – 01 – D

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B01D 37/00 (2006.01)

Patent

CA 2529385

A continuously operating bioreactor system combined with a filtration system is disclosed which may be directed to preparation and purifying of perfusion cell cultures. The bioreactor system comprises first reservior (100) containing fresh liquid medium (10) .the fresh liquid medium (10) is introduced into bioreactor (200) where the cultures are agitated by agitator (290) while they are mixed with cells introduced through inlet (250). The mixed liquid medium is then pumped to filtration reservoir comprising screen (330) which is rotated by rotator (315) from which retentate is recycled to bioreactor (200) .

L'invention concerne un système de bioréacteur fonctionnant en continu, combiné à un système de filtration et pouvant être destiné à la préparation et purification de culture cellulaires de perfusions. Ce système de bioréacteur comprend un premier réservoir (100) contenant un milieu liquide frais (10). Le milieu liquide frais (10) est introduit dans le bioréacteur (200) dans lequel les cultures sont agitées au moyen d'un agitateur (290) tout en étant mélangées avec des cellules introduites à travers un orifice d'entrée (250). Le milieu liquide mélangé est ensuite pompé dans un réservoir de filtration comprenant un écran (330) qui est mis en rotation par un élément rotateur (315) duquel le rétentat est recyclé vers le bioréacteur (200) .

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