Method and apparatus for heterogeneous chemiluminescence assay

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G01N 33/53 (2006.01) G01N 21/76 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/576 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2021586

Apparatus and method for performing a chemiluminescence assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

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