Method and apparatus for high performance separation by...

B - Operations – Transporting – 01 – D

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B01D 15/08 (2006.01) C07K 1/16 (2006.01)

Patent

CA 2059114

A high performance liquid chromatography method. The method employs an HPLC apparatus composed of a series of chromatography segments connected end-to-end by valved connectors which are operable to communicate adjacent segments, or to divert fluid from a joined segment to an elution collection site. In the method of the invention, a sample is applied to the segments, in an amount sufficient to saturate stationary-phase material in upstream and intermediate segments with bound sample compounds, with the most hydrophobic compounds saturating the stationary phase material in the most upstream segments. Elution of compounds from each segment individually yields at least one eluted sample with the desired compound in purified form.

L'invention est une méthode de chromatographie en phase liquide haute performance. Elle utilise un appareil composé d'une suite d'éléments de chromatographie connectés de bout en bout par des connecteurs vannés pouvant être actionnés pour mettre en communication des éléments adjacents ou pour faire dériver un fluide d'un élément joint à un site de collecte d'éluants. Dans la méthode de l'invention, un échantillon est introduit dans les éléments en quantité suffisante pour saturer la substance en phase stationnaire dans les éléments amont et intermédiaires avec des composés liés, les composés les plus hydrophobes saturant la substance en phase stationnaire dans les éléments les plus en amont. L'élution des composés provenant de chaque élément donne au moins dans chaque cas un échantillon élué avec le composé désiré sous forme purifiée.

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