Method and apparatus for identifying fluorophores

Details Method and apparatus for identifying fluorophores Method and apparatus for identifying fluorophores

Category

G - Physics

Subcategory

01

Subsubcategory

N

IPC codes

G01N 21/64 (2006.01)

Type

Patent

Patent number

CA 2237222

Description

The present invention is a unique method for identifying the presence, and preferably the identity, of a fluorophore by optically stimulating one or more fluorophores with an optical signal which has been modulated in intensity in the time domain. The stimulated fluorophore produces a resulting fluorescence which is demodulated to produce an electrical signal corresponding to the intensity modulation of the fluorescence. Finally, the electrical signal is compared to the modulation of the optical signal to determine whether or not the fluorophore is present. The present method can be used alone or in conjunction with known methods of optically analyzing fluorescence of fluorophores to determine the presence of fluorophores.

La présente invention se rapporte à un procédé unique d'identification de la présence, et de préférence de l'identité d'un fluorophore par stimulation optique d'un ou de plusieurs de ces fluorophores au moyen d'un signal optique qui a été modulé en intensité dans le domaine temporel. Le fluorophore stimulé produit une fluorescence qui est démodulée pour générer un signal électrique correspondant à la modulation de l'intensité de la fluorescence. Enfin le signal électrique est comparé à la modulation du signal optique ce qui permet de déterminer si il y a présence ou non du fluorophore. Ce procédé peut être utilisé seul ou associé à des procédés connus d'analyse optique de la fluorescence des fluorophores afin de déterminer la présence de ces derniers.

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