Method and apparatus for immobilized enzyme reactions

Details Method and apparatus for immobilized enzyme reactions Method and apparatus for immobilized enzyme reactions

C - Chemistry, Metallurgy

12

N

C12N 11/00 (2006.01) C12M 1/40 (2006.01) C12N 11/08 (2006.01) C12P 1/00 (2006.01)

Patent

CA 2102237

Perfusive matrices (10) useful in immobilized enzyme reactors (IMERs) are disclosed. The matrices define first and second interconnected sets of pores and an intraparticle high surface area (18) capable of immobilizing enzymes, in fluid communication with the second set of pores. The first and second sets of pores are embodied, for example, as the interstices (12) among particles and throughpores (14) within the particles. The particles also contain diffusive transport pores (16). The dimensions of the first and second sets of pores are such that, at fluid flow rates and pressure drops practical for immobilized enzyme reactions, convec- tive flow occurs in both pore sets, and the convective flow rate exceeds the rate of diffusion of the substrate and product to and from immobilized enzymes within the particles. This approach allows rapid enzyme loading and substrate conversion reactions at optimal catalytic flow rates.

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