Method and apparatus for in vitro reproduction and growth of...

Details Method and apparatus for in vitro reproduction and growth of... Method and apparatus for in vitro reproduction and growth of...

C - Chemistry, Metallurgy

12

N

195/145, 195/46

C12N 5/06 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12M 3/06 (2006.01)

Patent

CA 2011917

Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from said first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartments until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.

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