Method and apparatus for isolating platelets from blood

Details Method and apparatus for isolating platelets from blood Method and apparatus for isolating platelets from blood

A - Human Necessities

61

K

A61K 35/14 (2006.01) B01D 21/26 (2006.01) B01L 3/14 (2006.01)

Patent

CA 2483931

A platelet collection device comprising a centrifugal spin-separator container with a cavity having a longitudinal inner surface. A float in the cavity has a base, a platelet collection surface above the base, an outer surface. The float density is below the density of erythrocytes and above the density of plasma. The platelet collection surface has a position on the float which places it below the level of platelets when the float is suspended in separated blood. During centrifugation, a layer of platelets or buffy coat collects closely adjacent the platelet collection surface. Platelets are then removed from the platelet collection surface. Movement of a float having a density greater than whole blood through the sedimenting erythrocytes releases entrapped platelets, increasing the platelet yield.

L'invention concerne un dispositif de collecte de plaquettes comprenant un contenant de séparation par essorage centrifuge comportant une cavité à surface intérieure longitudinale. Dans la cavité, un flotteur est constitué d'une base, d'une surface de collecte de plaquettes et d'une surface extérieure. La densité du flotteur est inférieure à la densité des érythrocytes et supérieure à la densité du plasma. La surface de collecte des plaquettes se trouve dans une position sur le flotteur qui la place sous le niveau des plaquettes lorsque le flotteur est en suspension dans le sang séparé. Lors de la centrifugation, une couche de plaquettes ou une couche leuco-plaquettaire se crée adjacente à la surface de collecte des plaquettes. Les plaquettes sont alors retirées de la surface de collecte. Le déplacement d'un flotteur de densité supérieure au sang entier à travers les érythrocytes en sédimentation libère les plaquettes piégées, ce qui augmente le rendement en plaquettes.

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