Method and apparatus for maintenance and expansion of...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) C12N 5/071 (2010.01) C12N 5/0789 (2010.01) C12M 3/00 (2006.01) C12M 3/04 (2006.01) A61K 35/12 (2006.01) C12N 11/02 (2006.01) C12N 11/08 (2006.01)

Patent

CA 2360664

A method of expanding/maintaining undifferentiated hemopoietic stem cells or progenitor cells by obtaining undifferentiated hemopoietic stem cells or progenitor cells; and either seeding the undifferentiated hemopoietic stem cells or progenitor cells into a stationary phase plug-flow bioreactor in which a three-dimensional stromal cell culture has been pre-established on a substrate in the form of a sheet, the substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding/maintaining undifferentiated hemopoietic stem cells or progenitor cells, or culturing the undifferentiated hemopoietic stem cells or progenitor cells in conditioned medium obtained from such a reactor.

L'invention concerne une méthode de renouvellement/multiplication de cellules souches hématopoïétiques ou de progéniteurs indifférenciés, qui consiste à obtenir des cellules souches hématopoïétiques ou des progéniteurs indifférenciés ; puis soit à introduire ces cellules souches hématopoïétiques ou ces progéniteurs indifférenciés dans un bioréacteur à écoulement piston en phase stationnaire dans lequel une culture de cellules stromales tridimensionnelle a été préétablie sur un substrat se présentant sous la forme d'une feuille, ledit substrat comprenant une matrice fibreuse non tissée constituant un réseau de fibres tridimensionnel physiologiquement acceptable, ce qui permet le renouvellement/multiplication des cellules souches hématopoïétiques ou des progéniteurs indifférenciés, soit à cultiver les cellules souches hématopoïétiques ou les progéniteurs indifférenciés dans un milieu conditionné obtenu grâce audit réacteur.

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