C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/02 (2006.01) B01L 5/00 (2006.01) C12M 1/00 (2006.01) C12M 1/04 (2006.01) C12Q 1/26 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2487302
An apparatus and method for measuring nitric oxide production and oxygen consumption in cultures of adherent cells continuously and without destroying the cells. The method involves flowing growth media through a tube having adherent cells are adhered to the inner surface thereof and then contacting the growth media with an NO or O2 sensor to detect the concentration of NO or O2 in the growth media.
L'invention concerne un procédé et un appareil permettant de mesurer une production d'oxyde nitrique et une consommation d'oxygène dans des cultures de cellules adhérentes, de manière continuelle et sans détruire ces cellules. Ce procédé consiste à faire circuler un support de croissance à travers un tube présentant des cellules adhérentes collées à la surface de ce tube, puis à mettre en contact ce support de croissance avec un capteur de NO ou d'O¿2? pour détecter la concentration de NO ou d'O¿2? du support de croissance.
Baumgardner James E.
Otto Cynthia M.
Baumgardner James E.
Marks & Clerk
The Trustees Of The University Of Pennsylvania
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