Method and apparatus for measuring use of a substrate in a...

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/02 (2006.01) C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) G01N 27/42 (2006.01)

Patent

CA 2307603

The invention is a quantitative method for measuring the consump tion of a substrate in a microbially catalysed reaction. It involves the incubating of the sample substrate with an excess of a microorganism and an excess of a co-substrate until the substrate has been oxidised to a predetermined end point, and measuring the quantity of reduction of a co-substrate. The method can be used for the rapid determination of the BOD or the biological toxicity of the sample substrate.

Cette invention concerne un procédé quantitatif qui permet de mesurer la consommation d'un substrat dans une réaction catalysée de manière microbienne. Ce procédé consiste à incuber le substrat de l'échantillon avec une quantité excédentaire d'un micro-organisme et une quantité excédentaire d'un co-substrat. Cette incubation se poursuit jusqu'à oxydation dudit substrat en un point final prédéterminé, après quoi on mesure la quantité de réduction du co-substrat. Ce procédé peut être utilisé afin de déterminer rapidement la demande biochimique en oxygène (DBO) ou la toxicité biologique du substrat de l'échantillon.

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