G - Physics – 01 – N
Patent
G - Physics
01
N
73/124, 73/54, 1
G01N 21/17 (2006.01) C12M 1/34 (2006.01) G01N 21/33 (2006.01)
Patent
CA 1199812
ABSTRACT OF THE DISCLOSURE A method and apparatus are disclosed for detecting and iden- tifying microbial cells in a sample that may contain several differing microorganisms. The method comprises isolating one or more portions of the sample, combining respective sample portions with one or more materials known to be inhibiting reagents for the microbial cells to be detected, introducing an electrical potential across the respective sample portions, to induce an electrical charge across the surfaces of those microbial cells present, passing a beam of laser light through the sample portion for a period of time sufficient for the light to pass through the sample portion and be optically detected, measuring the birefringence effect of the sample portion, and comparing the birefringence effect with a con- trol sample portion containing the microbial cells under in- vestigation. The respective sample portions may be mixed with a series of different inhibiting reagents for the microbial cells under investigation, to develop a profile that can accurately identity the particular cells and segregate them from other bacteria that may appear in the same sample material. The apparatus comprises an electrical cell adapted to impose an electrical field across the sample under investigation, and an optical sensing assembly using a laser light source. An automated sample delivery system may be used, comprising one or more sample reservoirs fluidly connected to the inlet of the electrical cell, to sequentially transfer samples to the cell for examination.
426725
Lowke George E.
Meltzer Robert J.
Macrae & Co.
Warner-Lambert Company
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